The ERC PoC project STEDGate seeks to advance our understanding of neuronal connectivity and plasticity by developing STED-enabled holographic endo-nanoscopy for neuroscience. This ground-breaking technology promises atraumatic nanoscale invivo imaging of deep brain structures reaching depths up to 5 mm beneath the brain's surface. Collaborating with the start-up endeavour DeepEn, the team aims to facilitate the commercial transition of this technology.