Biodistribution and real-time monitoring of free or apoferritin-encapsulated charged cholinesterase reactivators

Oxime cholinesterase reactivators are used for life endangering intoxications caused by organophosphate agents. However, clinically used (so called charged reactivators) or experimental uncharged reactivators have very limited penetration to central nervous system, where organophosphates mediate irreversible changes in nerve tissue. For this reason, the various delivery systems are investigated to be able to encapsulate the oxime reactivators, help their blood-brain barrier permeation and release in the central nervous system for reactivation of organophosphate inhibited cholinesterases. This project focuses on traced oxime reactivators and their ferritin encapsulation. The free or ferritin encapsulated oxime reactivators and their release will be real-time monitored in vivo by using nuclear magnetic resonance (MRI)techniques.