Vladislav Krzyzanek, Ph.D.

Education:

1999: Ph.D., Technical University of Brno, Czech Republic

1995: university degree, Technical University of Brno, Czech Republic

Professional experience:

since 10/2011: research scientist, Institute of Scientific Instruments, The Czech Academy of Sciences, Brno, Czech Republic (since 01/2012 group leader of Microscopy for Biomedicine)

02/2001-09/2011: research scientist, Electron Microscopy and Analytics group, Institute of Medical Physics and Biophysics, University of M nster, Germany

03/2000-02/2001: guest scientist (NATO Science Programme Fellowship; Advanced), Electron Microscopy and Analytics group, Institute of Medical Physics and Biophysics, University of M nster, Germany

09/1999-03/2000: research scientist, Institute of Physical Engineering, Technical University of Brno, Czech Republic

11/1998-07/1999: guest scientist, Faculty of Physics, Warsaw University of Technology, Poland

09/1995-12/1998: scholarship, Institute of Physical Engineering, Technical University of Brno, Czech Republic

Professional affiliation:

• since 2009: Czechoslovak Microscopy Society (CSMS); president of CSMS 05/2019-12/2024
• since 2003: European Microscopy Society (EMS); president of EMS 2024-2028
• 2003-2013: German Society for Electron Microscopy (DGE)
• 2015-2018: Microscopy Society of America (MSA)

Primary research:

• quantitative imaging by STEM (mass-thickness measurement by electron scattering, 4D-STEM)
• low-temperature scanning electron microscopy (cryo-SEM)
• correlation of signals in electron microscopy
• links to academic profiles with more details:
  

Selected publications:

• Jilek Z. et al: Simulation Study of Low-Dose 4D-STEM Phase Contrast Techniques at the Nanoscale in SEM. Nanomaterials 15 (2025), 70.
• Skoupy R. et al: Robust Local Thickness Estimation of Sub-Micrometer Specimen by 4D-STEM. Small Methods 7 (2023), 2300258.
• Mrazova K. et al: Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cells. Microorganisms 11 (2023), 888.
• Szabova J. et al: Liposomal form of erlotinib for local inhalation administration and efficiency of its transport to the lungs. International Journal of Pharmaceutics 634 (2023), 122695.
• Vancova M. et al: Cathodoluminescence imaging of cellular structures labeled with luminescent iridium or rhenium complexes at cryogenic temperatures. Scientific Reports 12 (2022), 13432.
• Novackova I. et al: Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles. Polymers 14 (2022), 1761.
• Novackova I. et al: The role of polyhydroxyalkanoates in adaptation of Cupriavidus necator to osmotic pressure and high concentration of copper ions. International Journal of Biological Macromolecules 206 (2022), 977-989.
• Hanzelka P. et al: Low conductive thermal insulation pad with high mechanical stiffness. International Journal of Refrigeration 132 (2021), 92-99.
• Slouf M. et al: High Resolution Powder Electron Diffraction in Scanning Electron Microscopy. Materials 14 (2021), 7550.
• Kourilova X. et al: Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium Tepidimonas taiwanensis. Bioengineering 8 (2021), 141.
• Kourilova X. et al: The First Insight into Polyhydroxyalkanoates Accumulation in Multi-Extremophilic Rubrobacter xylanophilus and Rubrobacter spartanus. Microorganisms 9 (2021), 909.
• Slouf M. et al: Powder Nano-Beam Diffraction in Scanning Electron Microscope: Fast and Simple Method for Analysis of Nanoparticle Crystal Structure. Nanomaterials 11 (2021), 962.
• Kizovsky M. et al: Raman Microspectroscopic Analysis of Selenium Bioaccumulation by Green Alga Chlorella vulgaris. Biosensors 11 (2021), 115.
• Trudicova M. et al: Multiscale experimental evaluation of agarose-based semi-interpenetrating polymer network hydrogels as materials with tunable rheological and transport performance. Polymers 12 (2020), 2561.
• Monikh F.A. et al: Engineered Nanoselenium Supplemented Fish Diet: Toxicity Comparison with Ionic Selenium and Stability against Particle Dissolution, Aggregation and Release. Environmental Science: Nano 7 (2020), 2325-2336.
• Skoupy R. et al: Nanoscale estimation of coating thickness on substrates via standardless BSE detector calibration. Nanomaterials 10 (2020), 332.
• Pernicova I. et al: Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium. Polymers 12 (2020), 1235.
• Sedlacek P. et al: Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–2. Material Study on the Produced Copolymers. Polymers 12 (2020), 1298.
• Skoupy R. et al: Quantitative STEM imaging of electron beam induced mass loss of epoxy resin sections. Ultramicroscopy 202 (2019), 44-50.
• Knotigova P.T. et al: Application of Advanced Microscopic Methods to Study the Interaction of Carboxylated Fluorescent Nanodiamonds with Membrane Structures in THP-1 Cells: Activation of Inflammasome NLRP3 as the Result of Lysosome Destabilization. Molecular Pharmaceutics 16 (2019), 3441-3451.
• Sedlacek P. et al: PHA granules help bacterial cells to preserve cell integrity when exposed to sudden osmotic imbalances. New Biotechnology 49 (2019), 129-136.
• Sedlacek P. et al: What keeps polyhydroxyalkanoates in bacterial cells amorphous? A derivation from stress exposure experiments. Applied Microbiology and Biotechnology 103 (2019), 1905-1917.
• Hrubanova K. et al: Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy. Sensors 18 (2018), 4089.
• Hrubanova K. et al: The innovation of cryo-SEM freeze-fracturing methodology demonstrated on high pressure frozen biofilm. Micron 110 (2018), 28-35.
• Kucera D. et al: Characterization of the promising poly(3-hydroxybutyrate) producing halophilic bacterium Halomonas halophila. Bioresource Technology 256 (2018), 552-556.
• Slouf M. et al: Relations between morphology and micromechanical properties of alpha, beta and gamma phases of iPP. Polymer Testing 67 (2018), 522-532.
• Obruca S. et al: The presence of PHB granules in cytoplasm protects non-halophilic bacterial cells against the harmful impact of hypertonic environments. New Biotechnology 39 (2017), 68-80.
• Resch Y. et al: Molecular, structural and immunological characterization of Der p 18, a chitinase-like house dust mite allergen. PLoS ONE 11 (2016), e0160641.
• Obruca S. et al: Polyhydroxyalkanoates in bacterial cells – more than just storage materials. Materials Science Forum 851 (2016), e0157778.
• Obruca S. et al: How accumulation of poly(3-hydroxybutyrate) helps bacterial cells to survive freezing. PLoS ONE 11 (2016), e0157778.
• Mravec F. et al: Accumulation of PHA granules in Cupriavidus necator as seen by time-resolved confocal fluorescence microscopy. FEMS Microbiology Letters 363 (2016), fnw094.
• Tacke S. et al: A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics. Biophysical Journal 110 (2016), 758-765.
• Voberkova S. et al: Biofilm formation and extracellular polymeric substances (EPS), production by Bacillus subtilis depending on nutritional conditions in the presence of polyester film. Folia Microbiologica 61 (2016), 91-100.
• Bok J. et al: Measurements of current density distribution in shaped e-beam writers. Microelectronic Engineering 149 (2016), 117-124.
• Burdikova Z. et al: Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry. Dairy Science and Technology 95 (2015), 687-700.
• Samek O. et al: Identification of individual biofilm-forming bacterial cells using Raman tweezers. Journal of Biomedical Optics 20 (2015), 051038.
• Banerjee S. et al: Der p 11 is a Major Allergen for House Dust Mite-Allergic Patients Suffering from Atopic Dermatitis. Journal of Investigative Dermatology 135 (2015), 102-109.
• Bok J. et al: Effect of oxidation annealing on optical properties of YAG:Ce single crystals. Optical Materials 46 (2015), 591-595.
• Samek O. et al: Candida parapsilosis biofilm identification by raman spectroscopy. International Journal of Molecular Sciences 15 (2014), 23924-23935.
• Hajduova J. et al: Structure of polymeric nanoparticles in surfactant-stabilized aqueous dispersions of high-molar-mass hydrophobic graft copolymers. Colloids and Surfaces A: Physicochemical and Engineering Aspects 456 (2014), 10-17.
• Bernatova S. et al: Following the mechanisms of bacteriostatic versus bactericidal action using raman spectroscopy. Molecules 18 (2013), 13188-13199.
• Groscurth S. et al: Artificial Forisomes Are Ideal Models of Forisome Assembly and Activity That Allow the Development of Technical Devices. Biomacromolecules 13 (2012), 3076-3086.
• Hillebrand A. et al: Down-Regulation of Small Rubber Particle Protein Expression Affects Integrity of Rubber Particles and Rubber Content in Taraxacum brevicorniculatum. PLoS ONE 7 (2012) e41874.
• Porizka P. et al: Application of laser-induced breakdown spectroscopy to the analysis of algal biomass for industrial biotechnology. Spectrochimica Acta Part B: Atomic Spectroscopy 74-75 (2012), 169-176.
• Ernst A.M. et al: Sieve element occlusion (SEO) genes encode structural phloem proteins involved in wound sealing of the phloem. PNAS 109 (2012), E1980–E1989.
• Eissenberg J.C. et al: Drosophila GGA Model: An Ultimate Gateway to GGA Analysis. Traffic 12 (2011), 1821-1838.
• Krzyzanek V. et al: Polyelectrolyte multilayer capsules: Nanostructure and visualisation of nanopores in the wall. Soft Matter 7 (2011), 7034-7041.
• Kouyianou K. et al: The Chlorosome of Chlorobaculum tepidum: size, mass and protein composition revealed by electron microscopy, dynamic light scattering and mass spectrometry-driven proteomics. PROTEOMICS 11 (2011), 2867-2880.
• Pfaff M. et al: Low-energy electron scattering in carbon-based materials analyzed by scanning transmission electron microscopy and its application to sample thickness determination. Journal of Microscopy 243 (2011), 31-39.
• Edlmayr J. et al: Antibodies induced with recombinant VP1 from Human Rhinovirus exhibit cross-neutralization. European Respiratory Journal 37 (2011), 44-52.
• Krzyzanek V. et al: MASDET—A fast and user-friendly multiplatform software for mass determination by dark-field electron microscopy. Journal of Structural Biology 165 (2009), 78-87.
• Grote M. et al: Bundles of hexagonally arranged tubules in timothy grass pollen: Detection of a novel pollen component using anhydrous fixation and image analysis techniques in transmission electron microscopy. Journal of Microscopy 228 (2007), 34-39.
• Krzyzanek V. & Reichelt R.: High-Resolution Scanning Electron Microscope for Mass Determination: Progress in the Development of a New Nanoanalytical Tool. Microscopy & Microanalysis 13 (Suppl. 3), (2007), p. 80-81.
• Smarda J. et al: S-layers on cell walls of cyanobacteria. Micron 33 (2002), 257-277.
• Krzyzanek V.: Analysis of continuously distorted quasi-periodic images: Two-dimensional reconstruction of S layers of cyanobacteria. Optical Engineering 39 (2000), 872-878.
• Smajs D. et al: A comparative study of fine structure of cyanobacterial gas vesicles. Algological Studies 94 (1999), 305-316.
• Smajs D. et al: New findings of S layers among Cyanobacteria. Algological Studies 94 (1999), 317-332.
• Rachel R. et al: II. Fine structure of S-layers. FEMS Microbiology Reviews 20 (1997), 13-23.

Links:

• MASDET (software for mass determination by dark-field electron microscopy)
• MONCA (MATLAB package for Monte Carlo simulation of electron scattering in thin specimens)