Vladislav Krzyzanek, Ph.D.
Education:
1999: Ph.D., Technical University of Brno, Czech Republic
1995: university degree, Technical University of Brno, Czech Republic
Professional experience:
since 10/2011: research scientist,
Institute of Scientific Instruments,
The Czech Academy of Sciences,
Brno, Czech Republic (since 01/2012 group leader of Microscopy for Biomedicine)
02/2001-09/2011: research scientist,
Electron Microscopy and Analytics group,
Institute of Medical Physics and Biophysics,
University of Münster,
Germany
03/2000-02/2001: guest scientist (NATO Science Programme Fellowship; Advanced),
Electron Microscopy and Analytics group,
Institute of Medical Physics and Biophysics,
University of Münster,
Germany
09/1999-03/2000: research scientist,
Institute of Physical Engineering,
Technical University of Brno,
Czech Republic
11/1998-07/1999: guest scientist,
Faculty of Physics, Warsaw University of Technology, Poland
09/1995-12/1998: scholarship,
Institute of Physical Engineering,
Technical University of Brno,
Czech Republic
Professional affiliation:
Primary research:
- quantitative imaging by STEM (mass-thickness measurement by electron scattering)
- low-temperature scanning electron microscopy (cryo-SEM)
- correlation of signals in electron microscopy
- links to academic profiles with more details:
Selected publications:
- Trudicova M. et al:
Multiscale experimental evaluation of agarose-based semi-interpenetrating polymer network hydrogels as materials with tunable rheological and transport performance.
Polymers 12 (2020), 2561.
- Monikh F.A. et al:
Engineered Nanoselenium Supplemented Fish Diet: Toxicity Comparison with Ionic Selenium and Stability against Particle Dissolution, Aggregation and Release.
Environmental Science: Nano 7 (2020), 2325-2336.
- Skoupy R. et al:
Nanoscale estimation of coating thickness on substrates via standardless BSE detector calibration.
Nanomaterials 10 (2020), 332.
- Pernicova I. et al:
Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium.
Polymers 12 (2020), 1235.
- Sedlacek P. et al:
Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–2. Material Study on the Produced Copolymers.
Polymers 12 (2020), 1298.
- Skoupy R. et al:
Quantitative STEM imaging of electron beam induced mass loss of epoxy resin sections.
Ultramicroscopy 202 (2019), 44-50.
- Knötigova P.T. et al:
Application of Advanced Microscopic Methods to Study the Interaction of Carboxylated Fluorescent Nanodiamonds with Membrane Structures in THP-1 Cells: Activation of Inflammasome NLRP3 as the Result of Lysosome Destabilization.
Molecular Pharmaceutics 16 (2019), 3441-3451.
- Sedlacek P. et al:
PHA granules help bacterial cells to preserve cell integrity when exposed to sudden osmotic imbalances.
New Biotechnology 49 (2019), 129-136.
- Sedlacek P. et al:
What keeps polyhydroxyalkanoates in bacterial cells amorphous? A derivation from stress exposure experiments.
Applied Microbiology and Biotechnology 103 (2019), 1905-1917.
- Hrubanova K. et al:
Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy.
Sensors 18 (2018), 4089.
- Hrubanova K. et al:
The innovation of cryo-SEM freeze-fracturing methodology demonstrated on high pressure frozen biofilm.
Micron 110 (2018), 28-35.
- Kucera D. et al:
Characterization of the promising poly(3-hydroxybutyrate) producing halophilic bacterium Halomonas halophila.
Bioresource Technology 256 (2018), 552-556.
- Slouf M. et al:
Relations between morphology and micromechanical properties of alpha, beta and gamma phases of iPP.
Polymer Testing 67 (2018), 522-532.
- Obruca S. et al:
The presence of PHB granules in cytoplasm protects non-halophilic bacterial cells against the harmful impact of hypertonic environments.
New Biotechnology 39 (2017), 68-80.
- Resch Y. et al:
Molecular, structural and immunological characterization of Der p 18, a chitinase-like house dust mite allergen.
PLoS ONE 11 (2016), e0160641.
- Obruca S. et al:
Polyhydroxyalkanoates in bacterial cells – more than just storage materials.
Materials Science Forum 851 (2016), e0157778.
- Obruca S. et al:
How accumulation of poly(3-hydroxybutyrate) helps bacterial cells to survive freezing.
PLoS ONE 11 (2016), e0157778.
- Mravec F. et al:
Accumulation of PHA granules in Cupriavidus necator as seen by time-resolved confocal fluorescence microscopy.
FEMS Microbiology Letters 363 (2016), fnw094.
- Tacke S. et al:
A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics.
Biophysical Journal 110 (2016), 758-765.
- Voberkova S. et al:
Biofilm formation and extracellular polymeric substances (EPS), production by Bacillus subtilis depending on nutritional conditions in the presence of polyester film.
Folia Microbiologica 61 (2016), 91-100.
- Bok J. et al:
Measurements of current density distribution in shaped e-beam writers.
Microelectronic Engineering 149 (2016), 117-124.
- Burdikova Z. et al:
Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry.
Dairy Science and Technology 95 (2015), 687-700.
- Samek O. et al:
Identification of individual biofilm-forming bacterial cells using Raman tweezers.
Journal of Biomedical Optics 20 (2015), 051038.
- Banerjee S. et al:
Der p 11 is a Major Allergen for House Dust Mite-Allergic Patients Suffering from Atopic Dermatitis.
Journal of Investigative Dermatology 135 (2015), 102-109.
- Bok J. et al:
Effect of oxidation annealing on optical properties of YAG:Ce single crystals.
Optical Materials 46 (2015), 591-595.
- Samek O. et al:
Candida parapsilosis biofilm identification by raman spectroscopy.
International Journal of Molecular Sciences 15 (2014), 23924-23935.
- Hajduova J. et al:
Structure of polymeric nanoparticles in surfactant-stabilized aqueous dispersions of high-molar-mass hydrophobic graft copolymers.
Colloids and Surfaces A: Physicochemical and Engineering Aspects 456 (2014), 10-17.
- Bernatova S. et al:
Following the mechanisms of bacteriostatic versus bactericidal action using raman spectroscopy.
Molecules 18 (2013), 13188-13199.
- Groscurth S. et al:
Artificial Forisomes Are Ideal Models of Forisome Assembly and Activity That Allow the Development of Technical Devices.
Biomacromolecules 13 (2012), 3076-3086.
- Hillebrand A. et al:
Down-Regulation of Small Rubber Particle Protein Expression Affects Integrity of Rubber Particles and Rubber Content in Taraxacum brevicorniculatum.
PLoS ONE 7 (2012) e41874.
- Porizka P. et al:
Application of laser-induced breakdown spectroscopy to the analysis of algal biomass for industrial biotechnology.
Spectrochimica Acta Part B: Atomic Spectroscopy 74-75 (2012), 169-176.
- Ernst A.M. et al:
Sieve element occlusion (SEO) genes encode structural phloem proteins involved in wound sealing of the phloem.
PNAS 109 (2012), E1980–E1989.
- Eissenberg J.C. et al:
Drosophila GGA Model: An Ultimate Gateway to GGA Analysis.
Traffic 12 (2011), 1821-1838.
- Krzyzanek V. et al:
Polyelectrolyte multilayer capsules: Nanostructure and visualisation of nanopores in the wall.
Soft Matter 7 (2011), 7034-7041.
- Kouyianou K. et al:
The Chlorosome of Chlorobaculum tepidum: size, mass and protein composition revealed by electron microscopy, dynamic light scattering and mass spectrometry-driven proteomics.
PROTEOMICS 11 (2011), 2867-2880.
- Pfaff M. et al:
Low-energy electron scattering in carbon-based materials analyzed by scanning transmission electron microscopy and its application to sample thickness determination.
Journal of Microscopy 243 (2011), 31-39.
- Edlmayr J. et al:
Antibodies induced with recombinant VP1 from Human Rhinovirus exhibit cross-neutralization.
European Respiratory Journal 37 (2011), 44-52.
- Krzyzanek V. et al:
MASDET—A fast and user-friendly multiplatform software for mass determination by dark-field electron microscopy.
Journal of Structural Biology 165 (2009), 78-87.
- Grote M. et al:
Bundles of hexagonally arranged tubules in timothy grass pollen: Detection of a novel pollen component using anhydrous fixation and image analysis techniques in transmission electron microscopy.
Journal of Microscopy 228 (2007), 34-39.
- Krzyzanek V. & Reichelt R.:
High-Resolution Scanning Electron Microscope for Mass Determination: Progress in the Development of a New Nanoanalytical Tool.
Microscopy & Microanalysis 13 (Suppl. 3), (2007), p. 80-81.
- Smarda J. et al:
S-layers on cell walls of cyanobacteria.
Micron 33 (2002), 257-277.
- Krzyzanek V.:
Analysis of continuously distorted quasi-periodic images: Two-dimensional reconstruction of S layers of cyanobacteria.
Optical Engineering 39 (2000), 872-878.
- Smajs D. et al:
A comparative study of fine structure of cyanobacterial gas vesicles.
Algological Studies 94 (1999), 305-316.
- Smajs D. et al:
New findings of S layers among Cyanobacteria.
Algological Studies 94 (1999), 317-332.
- Rachel R. et al:
II. Fine structure of S-layers.
FEMS Microbiology Reviews 20 (1997), 13-23.
Links:
- MASDET (software for mass determination by dark-field
electron microscopy)
- MONCA (MATLAB package for Monte Carlo simulation of electron scattering
in thin specimens)