02109nas a2200277 4500008004100000022001400041245013300055210006900188300001200257490000800269520124000277653000701517653000701524100002101531700002301552700001901575700001901594700001501613700001901628700002501647700002201672700002201694700002101716700002201737856007201759 2022 eng d a0141-813000aThe role of polyhydroxyalkanoates in adaptation of Cupriavidus necator to osmotic pressure and high concentration of copper ions0 arole of polyhydroxyalkanoates in adaptation of Cupriavidus necat a977-9890 v2063 a

Polyhydroxyalkanoates (PHA) are abundant microbial polyesters accumulated in the form of intracellular granules by numerous prokaryotes primarily as storage of carbon and energy. Apart from their storage function, the presence of PHA also enhances the robustness of the microbial cells against various stressors. In this work, we investigated the role of PHA in Cupriavidus necator, a model organism concerning PHA metabolism, for adaptation to osmotic pressure and copper ions. In long-term laboratory evolution experiments, the bacterial culture was cultivated in presence of elevated doses of sodium chloride or copper ions (incubations lasted 78 passages for Cu2+ and 68 passages for NaCl) and the evolved strains were compared with the wild-type strain in terms of growth and PHA production capacity, cell morphology (investigated by various electron microscopy techniques), activities of selected enzymes involved in PHA metabolism and other crucial metabolic pathways, the chemical composition of bacterial biomass (determined by infrared and Raman spectroscopy) and also considering robustness against various stressors. The results confirmed the important role of PHA metabolism for adaptation to both tested stressors.

10aBF10aMF1 aNovackova, Ivana1 aHrabalova, Vendula1 aSlaninova, Eva1 aSedlacek, Petr1 aSamek, Ota1 aKoller, Martin1 aKrzyzanek, Vladislav1 aHrubanova, Kamila1 aMrazova, Katerina1 aNebesarova, Jana1 aObruca, Stanislav uhttps://www.sciencedirect.com/science/article/pii/S0141813022005712
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