02317nas a2200217   4500008004100000022001400041245010000055210006900155300000800224490000600232520167900238653000701917653000701924100002201931700002201953700001501975700002301990700002302013700002002036856004302056       2021                        eng d  a2076-260700aThe Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed Metschnikowia sp. Yeasts0 aUse of Raman Spectroscopy to Monitor Metabolic Changes in Stress  a2770 v93 a<p>Raman spectroscopy is a universal method designed for the analysis of a wide range of physical, chemical and biological systems or various surfaces. This technique is suitable to monitor various components of cells, tissues or microorganisms. The advantages include very fast non-contact and non-destructive analysis and no or minimal need for sample treatment. The yeasts Metschnikowia can be considered as industrially usable producers of pulcherrimin or single-cell lipids, depending on cultivation conditions and external stress. In the present study, Raman spectroscopy was used as an effective tool to identify both pulcherrimin and lipids in single yeast cells. The analysis of pulcherrimin is very demanding; so far, there is no optimal procedure to analyze or identify this pigment. Based on results, the strong dependence of pulcherrimin production on the ferric ion concentration was found with the highest yield in media containing 0.1 g/L iron. Further, production of lipids in Metschnikowia cells was studied at different temperatures and C:N ratios, using Raman spectroscopy to follow fatty acids composition, under different regimes, by monitoring the iodine number. The results of Raman spectroscopy were comparable with the fatty acid analysis obtained by gas chromatography. This study therefore supported use of Raman spectroscopy for biotechnological applications as a simple tool in the identification and analysis both the pulcherrimin and microbial lipids. This method provides a quick and relatively accurate estimation of targeted metabolites with minimal sample modification and allows to monitor metabolic changes over time of cultivation.</p>10aBF10aMF1 aNěmcová, Andrea1 aGonová, Dominika1 aSamek, Ota1 aSipiczki, Matthias1 aBreierová, Emilia1 aMárová, Ivana  uhttps://www.mdpi.com/2076-2607/9/2/27703030nas a2200217   4500008004100000022001400041245010800055210006900163300000900232490000600241520238000247653000702627653000702634100002202641700002302663700001502686700002302701700002302724700002002747856004502767       2021                        eng d  a2076-260700aUse of Waste Substrates for the Lipid Production by Yeasts of the Genus Metschnikowia—Screening Study0 aUse of Waste Substrates for the Lipid Production by Yeasts of th  a22950 v93 a<p>Oleogenic yeasts are characterized by the ability to accumulate increased amounts of lipids under certain conditions. These microbial lipids differ in their fatty acid composition, which allows them to be widely used in the biotechnology industry. The interest of biotechnologists is closely linked to the rising prices of fossil fuels in recent years. Their negative environmental impact is caused by significantly increased demand for biodiesel. The composition of microbial lipids is very similar to vegetable oils, which provides great potential for use in the production of biodiesel. In addition, some oleogenic microorganisms are capable of producing lipids with a high proportion of unsaturated fatty acids. The presented paper’s main aim was to study the production of lipids and lipid substances by yeasts of the genus Metschnikowia, to cultivate crude waste animal fat to study its utilization by yeasts, and to apply the idea of circular economy in the biotechnology of Metschnikowia yeasts. The work focuses on the influence of various stress factors in the cultivation process, such as reduced temperature or nutritional stress through the use of various waste substrates, together with manipulating the ratio of carbon and nitrogen sources in the medium. Yeast production properties were monitored by several instrumental techniques, including gas chromatography and Raman spectroscopy. The amount of lipids and in particular the fatty acid composition varied depending on the strains studied and the culture conditions used. The ability of yeast to produce significant amounts of unsaturated fatty acids was also demonstrated in the work. The most suitable substrate for lipid production was a medium containing glycerol, where the amount of accumulated lipids in the yeast M. pulcherrima 1232 was up to 36%. In our work, the crude animal fat was used for the production of high-value lipids, which to the best of our knowledge is a new result. Moreover, quantitative screening of lipase enzyme activity cultivated on animal fat substrate on selected yeasts of the genus Metschnikowia was performed. We found that for the yeast utilizing glycerol, animal fat seems to be an excellent source of carbon. Therefore, the yeast conversion of crude processed animal fat to value-added products is a valuable process for the biotechnology and food industry.</p>10aBF10aMF1 aNěmcová, Andrea1 aSzotkowski, Martin1 aSamek, Ota1 aCagáňová, Linda1 aSipiczki, Matthias1 aMárová, Ivana  uhttps://www.mdpi.com/2076-2607/9/11/229500798nas a2200241   4500008004100000245011300041210006900154300001400223490000800237653000700245653000700252100001900259700001900278700001300297700001500310700002200325700001700347700001900364700002000383700001400403700001600417856012300433       2019                        eng d00aWhat keeps polyhydroxyalkanoates in bacterial cells amorphous? A derivation from stress exposure experiments0 aWhat keeps polyhydroxyalkanoates in bacterial cells amorphous A   a1905-19170 v10310aBF10aMF1 aSedláček, P.1 aSlaninová, E.1 aEnev, V.1 aKoller, M.1 aNebesářová, J.1 aMárová, I.1 aHrubanová, K.1 aKrzyžánek, V.1 aSamek, O.1 aObruča, S.  uhttp://isibrno.cz/cs/what-keeps-polyhydroxyalkanoates-bacterial-cells-amorphous-derivation-stress-exposure-experiments00850nas a2200253   4500008004100000245014000041210006900181300000700250490000700257653000700264653000700271100001600278700001900294700001500313700002000328700002200348700001400370700001600384700002200400700001900422700001800441700001700459856012000476       2017                        eng d00aThe presence of PHB granules in cytoplasm protects non-halophilic bacterial cells against the harmful impact of hypertonic environments0 apresence of PHB granules in cytoplasm protects nonhalophilic bac  a100 v3910aBF10aMF1 aObruča, S.1 aSedláček, P.1 aMravec, F.1 aKrzyžánek, V.1 aNebesářová, J.1 aSamek, O.1 aKučera, D.1 aBenešová, Pavla1 aHrubanová, K.1 aMilerová, M.1 aMárová, I.  uhttp://isibrno.cz/cs/presence-phb-granules-cytoplasm-protects-non-halophilic-bacterial-cells-against-harmful-impact00698nas a2200217   4500008004100000245008600041210006900127300001300196490000700209653000700216100001600223700001900239700002000258700001500278700001900293700001400312700001600326700002200342700001700364856009900381       2016                        eng d00aAccumulation of Poly(3-hydroxybutyrate) Helps Bacterial Cells to Survive Freezing0 aAccumulation of Poly3hydroxybutyrate Helps Bacterial Cells to Su  ae01577780 v1110aMF1 aObruča, S.1 aSedláček, P.1 aKrzyžánek, V.1 aMravec, F.1 aHrubanová, K.1 aSamek, O.1 aKučera, D.1 aBenešová, Pavla1 aMárová, I.  uhttp://isibrno.cz/cs/accumulation-poly3-hydroxybutyrate-helps-bacterial-cells-survive-freezing00704nas a2200169   4500008004100000245019100041210006900232300001400301490000800315653000700323100001600330700001900346700001500365700001400380700001700394856012300411       2016                        eng d00aEvaluation of 3-hydroxybutyrate as an enzyme-protective agent against heating and oxidative damage and its potential role in stress response of poly(3-hydroxybutyrate) accumulating cells0 aEvaluation of 3hydroxybutyrate as an enzymeprotective agent agai  a1365-13760 v10010aMF1 aObruča, S.1 aSedláček, P.1 aMravec, F.1 aSamek, O.1 aMárová, I.  uhttp://isibrno.cz/cs/evaluation-3-hydroxybutyrate-enzyme-protective-agent-against-heating-and-oxidative-damage-and-its00686nas a2200193   4500008004100000245010700041210006900148300000700217490000700224653000700231100001900238700001400257700002000271700002200291700001700313700001700330700002000347856012500367       2016                        eng d00aMorphological and Production Changes in Stressed Red Yeasts Monitored Using SEM and Raman Spectroscopy0 aMorphological and Production Changes in Stressed Red Yeasts Moni  aS30 v2210aMF1 aHrubanová, K.1 aSamek, O.1 aHaroniková, A.1 aBernatová, Silva1 aZemánek, P.1 aMárová, I.1 aKrzyžánek, V.  uhttp://isibrno.cz/cs/morphological-and-production-changes-stressed-red-yeasts-monitored-using-sem-and-raman-spectroscopy00761nas a2200229   4500008004100000245010600041210006900147300000900216490000700225653000700232100001400239700001600253700001400269700001900283700002200302700001600324700001700340700001400357700002200371700001700393856012100410       2016                        eng d00aQuantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoates Produced by Cupriavidus necator H160 aQuantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoate  a18080 v1610aMF1 aSamek, O.1 aObruča, S.1 aŠiler, M1 aSedláček, P.1 aBenešová, Pavla1 aKučera, D.1 aMárová, I.1 aJežek, J1 aBernatová, Silva1 aZemánek, P.  uhttp://isibrno.cz/cs/quantitative-raman-spectroscopy-analysis-polyhydroxyalkanoates-produced-cupriavidus-necator-h1600720nas a2200205   4500008004100000245011700041210006900158300001200227490000700239653000700246100002000253700001900273700001400292700001600306700001700322700002200339700001400361700001700375856012200392       2015                        eng d00aCryo-SEM and Raman Spectroscopy Study of the Involvement of Polyhydroxyalkanoates in Stress Response of Bacteria0 aCryoSEM and Raman Spectroscopy Study of the Involvement of Polyh  a183-1840 v2110aMF1 aKrzyžánek, V.1 aHrubanová, K.1 aSamek, O.1 aObruča, S.1 aMárová, I.1 aBernatová, Silva1 aŠiler, M1 aZemánek, P.  uhttp://isibrno.cz/cs/cryo-sem-and-raman-spectroscopy-study-involvement-polyhydroxyalkanoates-stress-response-bacteria00729nas a2200205   4500008004100000245011800041210006900159300001400228490000700242653000700249100001400256700002000270700002200290700001900312700001400331700001700345700002000362700001700382856012400399       2015                        eng d00aSEM and Raman Spectroscopy Applied to Biomass Analysis for Application in the Field of Biofuels and Food Industry0 aSEM and Raman Spectroscopy Applied to Biomass Analysis for Appli  a1775-17760 v2110aMF1 aSamek, O.1 aHaroniková, A.1 aVaškovicová, N.1 aHrubanová, K.1 aJežek, J1 aMárová, I.1 aKrzyžánek, V.1 aZemánek, P.  uhttp://isibrno.cz/cs/sem-and-raman-spectroscopy-applied-biomass-analysis-application-field-biofuels-and-food-industry-0